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丹参酮ⅡA 对血管平滑肌细胞周期调控因子CyclinD1 和 CDK4 表达的影响

发布日期:2012-11-27 
丹参酮ⅡA对血管平滑肌细胞周期调控因子CyclinD1 和 CDK4 表达的影响

【作者】 韩芬Han Fen1; 杨晓Yang Xiao2; 沈晓君Shen Xiaojun2;
【机构】 1.郑州铁路职业技术学院; 河南 郑州 450052Zhengzhou Railroad Occupation TechnicalCollege; Zhengzhou; Henan; China 4500522.河南中医学院; 河南 郑州 450046H enan University ofTraditionalChinese Medicine; China 450046; 【摘要】 摘要:目的:研究丹参酮ⅡA 拮抗同型半胱氨酸诱导的增殖血管平滑肌细胞周期的调控机制。方法:建立 H CY 诱导的兔VSM C 增殖模型,与不同浓度的丹参酮ⅡA 共同培养 48 h 后,用流式细胞仪分析 VSM C 细胞周期;检测细胞周期调控因子 Cy-clinD1 和 CDK4 的表达。结果:0.25 m g L 1、0.50 m g L 1、1.00 m g L 1丹参酮ⅡA 组 S 期细胞数明显减少,G0/G1期细胞数明显增多,与 H CY 组比较,差异有统计学意义(P < 0.01);CyclinD1 和 CDK4 的表达量均随丹参酮ⅡA 剂量增加而减少。结论:丹参酮ⅡA 拮抗 H CY 诱导的兔 VSM C 增殖,其主要机制可能与 CyclinD1 及 CDK4 的低表达所致的细胞周期抑制有关。A bstract:Objective:To study the m echanism oftanshinoneⅡA in the regulation ofvascular sm ooth m uscle cellinduced by hom ocys-teine.M ethods:Establish VSM C m ultiplicative m odelofrabbits induced by H CY.Use flow cytom eterto analyze VSM C m itotic cycle af-ter48h with differentconcentration oftanshinone ⅡA.Detectregulating factor CyclinD1 and CDK4 expression ofm itotic cycle.Rre-sults:The S phase cellnum bersignificantly reduced and G0/G1phase cellnum ber obviously increased in 0.25 m g L 1,0.5 m gL 1,1 m g L 1tanshinoneⅡA group.The difference was significantcom pared with H CY group(P < 0.01).The CyclinD1 and CDK4expression am ountdecreased following the increase of tanshinoneⅡA.Conclusion:The m ain m echanism of TanshinoneⅡA againstVSM C proliferation induced by H CY is possible referring to m itotic cycle inhibition caused by CyclinD1 and CDK4 low expression  
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